報(bào)告題目：Droplet Microfluidics for High Throughput Single-Cell Analysis with Single-Molecule Sensitivity
報(bào) 告 人：楊朝勇 教授（廈門大學(xué)）
報(bào)告時(shí)間：2015年5月9日下午2：30
報(bào)告地點(diǎn)：化學(xué)樓二樓一號(hào)會(huì)議室
報(bào)告人簡(jiǎn)介：
楊朝勇，男，廈門大學(xué)特聘教授、博士生導(dǎo)師、固體表面物理化學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室固定成員、廈門大學(xué)化學(xué)化工學(xué)院化學(xué)生物學(xué)系副系主任。相繼于1998和2001年在廈門大學(xué)化學(xué)系獲得學(xué)士與碩士學(xué)位，2006年獲得美國(guó)佛羅里達(dá)大學(xué)博士學(xué)位，2006年9月至2007年12月在美國(guó)加州大學(xué)伯克利分校從事博士后研究。主要研究領(lǐng)域?yàn)樯锓治龌瘜W(xué)，在分子探針、微流控芯片、信號(hào)放大、單細(xì)胞分析、食品安全等方向取得了創(chuàng)新性的成果。 在PNAS、JACS、Angew Chem Int Ed、Anal Chem等學(xué)術(shù)刊物上發(fā)表論文100多篇，H index 31。研究工作被美國(guó)Washington Times、The Scientist、英國(guó)RSC Chemistry World、歐洲Wiley Chemistry Views、JACS Spotlights、光明日?qǐng)?bào)等媒體與刊物做了專題報(bào)道。先后獲得了美國(guó)化學(xué)會(huì)分析化學(xué)研究生獎(jiǎng)、中國(guó)政府國(guó)家優(yōu)秀自費(fèi)留學(xué)生獎(jiǎng)、中美化學(xué)與化學(xué)生物學(xué)教授協(xié)會(huì)杰出教授獎(jiǎng)、中國(guó)青年分析化學(xué)家獎(jiǎng)、福建省五四青年獎(jiǎng)?wù)隆⒏＝ㄊ∵\(yùn)盛青年科技獎(jiǎng)、福建省高校領(lǐng)軍人才等獎(jiǎng)項(xiàng)。2010年獲福建省杰出青年科學(xué)基金, 2013年獲國(guó)家基金委杰出青年基金。承擔(dān)973計(jì)劃課題1項(xiàng)、國(guó)家自然科學(xué)基金5項(xiàng)。現(xiàn)任《分析化學(xué)》、Analyst、Biomicrofluidics等期刊編、BMC Biochemistry 副主編。
報(bào)告簡(jiǎn)介：
It is well established that individual cells, even from the same origin, differ from each other in many aspects due to stochastic biological processes and differences in environmental perturbations. Cell heterogeneity has been found to play an important role in many biological processes, including cellular differentiation and immune response, as well as disease development. The heterogeneity of cells in culture and in organisms poses a challenge for many experimental measurements. Traditional ensemble analysis based on averaging a large population of cells, as a result, masks the behavior of minority subpopulations and effectively blinding researchers to possibly interesting differences between cells. Single-cell analysis is an important and emerging field that gives insights into heterogeneity between cells and advanced cellular processes at high resolution, which is important for cancer research, regenerative medicine, immune system research and diagnostics, as well as for the production of therapeutics. Microfluidics has proven to be a leading tool for single cell analysis since device dimensions are on the same scale as those of cells, allowing for precise fluid and cell manipulation at high throughput. In this talk, I will present our recent efforts on developing droplet microfluidic technology for high throughput single cell isolation, manipulation, and analysis at the DNA, RNA and protein level with single-molecule sensitivity.
