報告題目：基因進化在化學中的應用
報 告 人：王江云研究員
報告時間：2018年11月7日（周三）晚上7:00
報告地點：化學樓一號會議室
邀 請 人：劉紅梅教授
報告人簡介：
王江云，中國科學院生物物理研究所研究員，國家杰出青年基金獲得者，教育部青年長江學者。2003年獲美國伊利諾伊大學厄巴納-香檳分校博士學位，2003-2007年在The Scripps Research Institute從事博士后研究。2008年到中國科學院生物物理研究所工作，組建了以研究生物物理化學、合成生物學及化學生物學為主要方向的課題組，主要研究內容包括：1. 發展蛋白質及RNA標記的新方法，研究蛋白質及RNA的定位、結構、折疊以及相互作用。2. 研究含有金屬卟啉和銅離子的蛋白質的結構功能，并在此基礎上設計新的金屬酶。在國際一流期刊如Nat. Chem., Nat. Chem. Bio., Nat. Commun., JACS, Angew. Chem. Int. Ed.等上發表重要學術論文；在一些重要的國際會議如International Conference on Bioinorganic chemistry, Asian Biological Inorganic Chemistry Conference, IUPAC Chemical Biology Conference等上作大會報告或邀請報告；獲得第五屆中國化學會-英國皇家化學會青年化學獎、第十三屆中國青年科技獎。
報告簡介：
Photosensitizers, which harness light energy to upgrade weak reductants to strong reductants, are pivotal components of the natural and artificial photosynthesis machineries. However, it has proved difficult to enhance and expand their functions through genetic engineering. Here we report a genetically encoded, 27 kD photosensitizer protein (PSP), which facilitates the rational design of miniature photocatalytic CO2 reducing enzymes. Visible light drives PSP efficiently into a long-lived triplet excited state (PSP*), which reacts rapidly with NADH to generate a super-reducing radical (PSP?), which is strong enough to reduce many CO­2 reducing catalysts. We determined the 3D structure of PSP? at 1.8 Å resolution by X-ray crystallography. Genetic engineering enabled the site-specific attachment of a nickel-terpyridine complex, and modular optimization of the photochemical properties of PSP, chromophore/catalytic center distance and catalytic center microenvironment, culminating in a miniature photocatalytic CO2 reducing enzyme which has a CO2/CO conversion quantum efficiency of 2.6%.
